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Water pollution, the release of substances into subsurface groundwater or into lakes, streams, rivers, estuaries, and oceans to the point where the substances interfere with beneficial use of the water or with the natural functioning of ecosystems. In addition to the release of substances, such as chemicals or microorganisms, water pollution may also include the release of energy, in the form of radioactivity or heat, into bodies of water.
Water pollution still would have occurred even without those factors though. And microorganisms have always been in water since the world created the first microbes