The initial graph reading of the DNS reduction at 540nm over a 5-minute time period, at 30 second intervals.

(4 transformants/0.1µg) x (1ml/0.25ml) = 160 transformants per µg.

LB Amp -has no growth under UV light, nor is there a lawn.

This is because the untransformed bacteria are unable to grow in the presence of the antibiotic since bacterial cell walls are unable to form and the bacterial cells die.

LB -has no visible growth under UV light, but has a visible lawn when viewed by the naked eye.

This could also be due to the fact that this is a non-selective agar plate.

Thus, untransformed bacterial cells will cover the plate forming a lawn, which is cream in colour.

Graph showing the Spectrophotometric reading of the DNA isolated through this protocol.

Spectrophotometric reading of the DNA isolated through this protocol.

The ladder showing the number of kilobases per line on the ladder.

The absorbance readings obtained for each of the dilutions at an Absorbance of 550nm.

The green dye beginning to elute from the ion-exchange column.

This indicates that the green dye is acationic and binds more readily in the column.

The suspension plate shows that at well 1 there is no growth, thus showing the negative control is successful.

At well 2 we have a 16mm ring, showing the greatest amount of lysozyme activity occurred here.

At well 3 we have a 14mm ring, showing that lysozyme activity still occurs at half its concentration.

At well 4 we see an 11mm ring, indicating that at even low concentrations lysozyme activity is still able to take place.

The ELISA antigen test showing the test for sleep deprivation in drivers.

A is the positive control of a non-sleep deprived individual.

Number 2 (from the left) is the negative control, showing the result of a heavily sleep deprived individual.

Number 3 (from the left) is person number A, who is sleep deprived.

Number 4 (from the left) shows person number B, who is heavily sleep deprived.

Figue 11: The SDS page gel showing (from left to right) the molecular weights of Lysozyme, the ladder, Yeast extract and Albumin.

From figure 10 we can surmise that the lysozyme is approximately 140kb in weight, while yeast extract contains several proteins ranging from 250kb to 15 kb in weight.

The albumin serum is about 40kb in weight.

The absorbance readings of glucose and sucrose at 540nm for each of the 8 groups, our groups results are in column 8.

All these collections are taken from online. If there is any mistake, you will be forgiven. Thanks.